Enzyme-linked immunosorbent assays (ELISA) complement antibody specificity with the general enzyme sensitivity assay. This is done using an easy-to-perform test to detect antibodies or antigens. Antibody or antigen concentration is measured by ELISA. If you are looking for Human PLAT/TPA ELISA Kit Price PicoKine visit BosterBio.

MPO ELISA kit is also one of the best kits available in the market.

There are differences in these methods:

• ELISA can detect the presence of antigens recognized by antibodies.

• Used to test for antibodies that recognize antigens in samples.

• Several combination antibodies and antigen detection kits are also available.

Steps for ELISA Process:

1. Primary antigen (or antibody) is used to cover the pores of the microtiter plate

2. Plates are washed to remove unbound antibodies. False-positive results are minimized by blocking unrelated sites (usually with bovine serum albumin or other animal proteins).

3. Addition of selected enzyme-conjugated primary detection antibodies.

4. Addition of an enzyme-conjugated secondary antibody (eg anti-mouse IgG) causes this enzyme to react with any substrate to produce a colored product indicating a positive reaction.

The results were measured by a spectrophotometer. Some analyzes include a sample monitoring system that shows color changes at each step of the process. This can be important when using an automated liquid purification system because it can indicate at which stage the process is being interrupted or that no reagent has been added.